LIMK1 T508EE
(Plasmid
#245950)
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PurposeExpressing constitutively active mimetic of phospho-Thr508 LIM Kinase 1
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Depositing Lab
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Depositing OrganizationUniversity of California, San Diego (UCSD)
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 245950 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 245950-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 245950-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerUnknown
- Backbone size w/o insert (bp) 5428
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLIM domain kinase 1
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Alt nameLimk1
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SpeciesM. musculus (mouse)
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MutationT508EE (insert of 2 glutamates)
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Entrez GeneLimk1 (a.k.a. KIZ-1, LIMK-1, Limk)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unspecified (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Constitutively active LIMK1 increases the non-actin-binding phospho-cofilin in cells. Phospho-Ser3-cofilin is an activator of phospholipase D1.
DNA (Catalog # 245950-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 245950-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LIMK1 T508EE was a gift from Gordon Gill (Addgene plasmid # 245950 ; http://n2t.net/addgene:245950 ; RRID:Addgene_245950) -
For your References section:
Structural features of LIM kinase that control effects on the actin cytoskeleton. Edwards DC, Gill GN. J Biol Chem. 1999 Apr 16;274(16):11352-61. doi: 10.1074/jbc.274.16.11352. 10.1074/jbc.274.16.11352 PubMed 10196227
