VC 438: pAAV-hSyn-ASIP-T2A-eGFP
(Plasmid #246599)

• Purpose: AAV expression of Agouti gene from African striped mice (Rhabdomys pumilio) and EGFP from hSyn promoter
• Depositing Lab: Catherine Peña
• Publication: Rogers et al Nature. 2026 Apr;652(8110):694-702. doi: 10.1038/s41586-026-10123-4. Epub 2026 Feb 18.

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Backbone

• Vector backbone: pAAV
• Backbone manufacturer: James M. Wilson (Addgene # 105539)
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Agouti
• Alt name: ASIP
• Alt name: a
• Species: Rhabdomys pumilio
• GenBank ID: GCA_030674055.1
• Promoter: hSyn
• Tag / Fusion Protein:
  • T2A-EGFP (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Rhabdomys Agouti coding exons 2-4 (Accession JANHMN010000002) were cloned by the Princeton Neuroscience Institute Viral Core Facility at Princeton University.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2025.06.17.658598 for bioRxiv preprint.