pCS2 xenopus sizzled
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24684||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesX. laevis (frog)
Insert Size (bp)1200
Entrez Geneszl (a.k.a. xszl)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Best in situ probe.
For anitsense: linearize with NotI
Transcribe with SP6
For mRNA injections use 200 pg to dorsalize.
E248D does not affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS2 xenopus sizzled was a gift from Edward De Robertis (Addgene plasmid # 24684 ; http://n2t.net/addgene:24684 ; RRID:Addgene_24684)
For your References section:Embryonic dorsal-ventral signaling: secreted frizzled-related proteins as inhibitors of tolloid proteinases. Lee HX, Ambrosio AL, Reversade B, De Robertis EM. Cell. 2006 Jan 13. 124(1):147-59. 10.1016/j.cell.2005.12.018 PubMed 16413488