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pcDNA3 xDarmin
(Plasmid #24691)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24691 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Darmin
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    2900
  • Entrez Gene
    darmin (a.k.a. endocut, MGC68854)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For antisense: linearize with BamHI. Transcribe with SP6.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 xDarmin was a gift from Edward De Robertis (Addgene plasmid # 24691)

  • For your References section:

    Darmin is a novel secreted protein expressed during endoderm development in Xenopus. Pera EM, Martinez SL, Flanagan JJ, Brechner M, Wessely O, De Robertis EM. Gene Expr Patterns. 2003 May . 3(2):147-52. 10.1016/S1567-133X(03)00011-5 PubMed 12711541
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