|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24697||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesX. laevis (frog)
Insert Size (bp)1485
/ Fusion Protein
- HA (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
For sense linearize with NotI, transcribe with SP6.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS2 xCrescent-HA was a gift from Edward De Robertis (Addgene plasmid # 24697 ; http://n2t.net/addgene:24697 ; RRID:Addgene_24697)
For your References section:A direct screen for secreted proteins in Xenopus embryos identifies distinct activities for the Wnt antagonists Crescent and Frzb-1. Pera EM, De Robertis EM. Mech Dev. 2000 Sep . 96(2):183-95. 10.1016/S0925-4773(00)00394-4 PubMed 10960783
Map uploaded by the depositor.