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pAcGFP-N1_CBPIDR6shuffle
(Plasmid #247245)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247245 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pAcGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4726
  • Total vector size (bp) 12052
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CREB binding protein (CBP)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    7326
  • Mutation
    Amino acids within the IDR6 region, CBP aa 1856 - 2090, have been shuffled, where the overall sequence composition was maintained but the sequence was randomly shuffled to disrupt the sequence patterning.
  • Entrez Gene
    CREBBP (a.k.a. CBP, KAT3A, MKHK1, RSTS, RSTS1)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.06.04.597392 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAcGFP-N1_CBPIDR6shuffle was a gift from Daniel Bose (Addgene plasmid # 247245 ; http://n2t.net/addgene:247245 ; RRID:Addgene_247245)

  • For your References section:

    CBP-IDRs regulate acetylation and gene expression. Gelder KL, Carruthers NA, Gilbert G, Harrison LJ, Evans BV, Evans TI, Ball SS, Dunning M, Craggs TD, Twelvetrees AE, Bose DA. Cell Rep. 2026 Mar 12;45(3):117109. doi: 10.1016/j.celrep.2026.117109. 10.1016/j.celrep.2026.117109 PubMed 41824454
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