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pKMDDV_Plasmid_2b
(Plasmid #249057)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249057 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pJF143.J3
  • Backbone size w/o insert (bp) 4431
  • Total vector size (bp) 5716
  • Modifications to backbone
    Additional constitutive scRNA_GFP and GFP
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    scRNA
  • Species
    Synthetic
  • Insert Size (bp)
    250
  • Promoter ptrc

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Fast Folding Green Fluorescent Protein
  • Alt name
    ff GFP
  • Alt name
    GFP
  • Species
    Synthetic
  • Insert Size (bp)
    1000
  • Promoter BBa_J23117

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKMDDV_Plasmid_2b was a gift from Domitilla Del Vecchio (Addgene plasmid # 249057 ; http://n2t.net/addgene:249057 ; RRID:Addgene_249057)

  • For your References section:

    Resource competition shapes CRISPR-mediated gene activation. Aravind KM, Del Vecchio D. Cell Systems, 2026; 17 10.1016/j.cels.2025.101511
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