zPRL3_1-169_C104D_pET-15b
(Plasmid
#250560)
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PurposeBacterial expression of zebrafish PRL3/PTP4A3 C104D
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250560 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepET-15b
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namezebrafish PRL3
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Alt namePTP4A3
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SpeciesD. rerio (zebrafish)
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MutationCysteine 104 to Aspartic acid (C104D)
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Entrez Geneptp4a3a (a.k.a. ptp4a3, wu:fc54b05, wu:fv52d11, zgc:77109)
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Tag
/ Fusion Protein
- His-tag (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
zPRL3_1-169_C104D_pET-15b was a gift from Kalle Gehring (Addgene plasmid # 250560 ; http://n2t.net/addgene:250560 ; RRID:Addgene_250560)