pUBC-SV40NLS-PopZ3H-QtE-TSapphire
(Plasmid #250610)

• Purpose: Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 50nm nucQtE-GEMs under the UBC promoter
• Depositing Lab: Liam Holt
• Depositing Organization: NYU Grossman School of Medicine
  Located in United States of America
• Publication: Herzog et al Mol Cell. 2026 Mar 5;86(5):817-833.e7. doi: 10.1016/j.molcel.2026.02.005.

Backbone

• Vector backbone: lentiviral vector
• Total vector size (bp): 9342
• Vector type: Mammalian Expression, Bacterial Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Encapsulin
• Species: Quasibacillus thermotolerans
• Insert Size (bp): 846
• Mutation: SV40NLS signal on N-terminal
• Tags / Fusion Proteins:
  • TSapphire (C terminal on insert)
  • PopZTag (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CCAAAAAAGAAGAGAAAGGTCTCTAGAGAGGTCGCAGAGCAGCTGGTT

Resource Information

• A portion of this plasmid was derived from a plasmid made by: SV40NLS was cloned into Addgene #240101 by Cindy Hernandez

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2025.06.20.660750 for our bioRxiv preprint.