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pET22b-OleTLA
(Plasmid #251243)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251243 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET22b
  • Backbone size w/o insert (bp) 5367
  • Total vector size (bp) 6639
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OleTLA
  • Species
    Lacicoccus alkaliphilus
  • Insert Size (bp)
    1272
  • GenBank ID
    PQ308727.1
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET22b-OleTLA was a gift from Pimchai Chaiyen (Addgene plasmid # 251243 ; http://n2t.net/addgene:251243 ; RRID:Addgene_251243)

  • For your References section:

    Unique structural features define the decarboxylation activity of a CYP152 fatty acid decarboxylase from Lacicoccus alkaliphilus. Phaisan S, Phintha A, Trisrivirat D, Lawan N, Sucharitakul J, Charoenpol A, Watthaisong P, Tanaka H, Kurisu G, Chaiyen P. J Biol Chem. 2025 Jul;301(7):110397. doi: 10.1016/j.jbc.2025.110397. Epub 2025 Jun 19. 10.1016/j.jbc.2025.110397 PubMed 40543591
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