pSnH-OROV-M
(Plasmid
#252623)
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PurposeRecombinant expression of codon-optimised OROV M segment with N-terminal HA tag.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252623 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepcDNA3.1
- Total vector size (bp) 9646
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOropouche virus M segment
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Alt nameGn
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Alt nameGc
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Alt nameNSm
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SpeciesOropouche virus
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Insert Size (bp)4293
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GenBank IDKP052851.1
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Tag
/ Fusion Protein
- HA tag between signal sequence and start of Gn (N terminal on insert)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSnH-OROV-M was a gift from Stephen Graham (Addgene plasmid # 252623 ; http://n2t.net/addgene:252623 ; RRID:Addgene_252623) -
For your References section:
Oropouche Virus Glycoprotein Topology and Cellular Requirements for Glycoprotein Secretion. Barbosa NS, Concha JO, daSilva LLP, Crump CM, Graham SC. J Virol. 2023 Jan 31;97(1):e0133122. doi: 10.1128/jvi.01331-22. Epub 2022 Dec 8. 10.1128/jvi.01331-22 PubMed 36475765