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pTrcHisbglk
(Plasmid #252958)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252958 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pTrcHis
  • Backbone manufacturer
    Thermo Fisher
  • Backbone size w/o insert (bp) 4414
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    beta glucoside kinase
  • Species
    Klebsiella pneumoniae
  • GenBank ID
    AY035305.1
  • Promoter trc
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    John Thompson from the National Institute of Dental and Craniofacial Research (NIDCR), a branch of the U.S. National Institutes of Health.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTrcHisbglk was a gift from John Thompson (Addgene plasmid # 252958 ; http://n2t.net/addgene:252958 ; RRID:Addgene_252958)

  • For your References section:

    Beta-glucoside kinase (BglK) from Klebsiella pneumoniae. Purification, properties, and preparative synthesis of 6-phospho-beta-D-glucosides. Thompson J, Lichtenthaler FW, Peters S, Pikis A. J Biol Chem. 2002 Sep 13;277(37):34310-21. doi: 10.1074/jbc.M206397200. Epub 2002 Jul 10. 10.1074/jbc.M206397200 PubMed 12110692
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