pLJSRSF7-T7-6xHis-SUMO-MRT-CBD
(Plasmid #253350)

• Purpose: For the expression of group II intron encoded reverse transcriptase MarathonRT with N-terminal SUMO-tag and C-terminal CBD-tag in E. coli.
• Depositing Lab: Peter Sarin
• Publication: Pedor et al Bio-protocol 2026, 16(11): e5703. DOI: 10.21769/BioProtoc.5703.

Backbone

• Vector backbone: pLJSRSF7
• Backbone manufacturer: Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1046-5928(15)30039-5. doi: 10.1016/j.pep.2015.08.019.
• Backbone size w/o insert (bp): 3718
• Total vector size (bp): 5359
• Modifications to backbone: Removal of fragment NcoI (85)-BamHI (1695). Replaced BamHI (89)- KpnI (411) with insert.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 6xHis-SUMO-MarathonRT with added C-terminal CBD-tag originating from the backbone.
• Alt name: MRT-CBD
• Species: Eubacterium rectale (Eu.re.I2)
• Insert Size (bp): 1640
• Promoter: T7
• Tags / Fusion Proteins:
  • 6xHis-SUMO (N terminal on insert)
  • CBD (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Backbone from Addgene plasmid #64693; publication: Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1046-5928(15)30039-5. doi: 10.1016/j.pep.2015.08.019. Insert from Addgene plasmid #109029; publication: Zhao et al RNA. 2018 Feb;24(2):183-195. doi: 10.1261/rna.063479.117. Epub 2017 Nov 6.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry