pJH4782
(Plasmid #253828)

• Purpose: Pcex-1 RFP cex-1 3' UTR C.elegans cex-1 transcriptional report
• Depositing Lab: Mei Zhen
• Publication: Three Muscle-Specific DAF-16/FOXO Transcriptional Targets Activated by Reduced Insulin/IGF-1 Signaling (unpublished)

Backbone

• Vector backbone: pBSK
• Backbone size w/o insert (bp): 6500
• Total vector size (bp): 7535
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cex-1 3'UTR
• Species: C. elegans (nematode)
• Insert Size (bp): 1035
• Promoter: Pcex-1
• Tag / Fusion Protein:
  • RFP

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AAGCTTGGGCTGCAgcatgcggccgccaccaacctggaaaactaca
• 3′ sequencing primer: TCACCGAAACGCGCGAGACGAAAGGGCCCttaaattcattttattatct

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit for https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 bioRxiv preprint.

There is a single nt discrepancy in Pgpc-1. This does not affect the plasmid's function.

The SNPs in pcex-1 do not change the expression pattern of Pcex-1. We observed pattern expression in RIM interneuron only. In addition, the SNP in worm mCherry also does not affect the expression or fluorescence of the RFP.