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pET15Sumo2-HRV2-3CLpro_GTM_001
(Plasmid #254044)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254044 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET15Sumo2_NESG
  • Backbone manufacturer
    Novagen/Northeast Structural Genomics Consortium
  • Backbone size w/o insert (bp) 5680
  • Total vector size (bp) 6226
  • Modifications to backbone
    An N-terminal 6xHis tag fused to the Smt3 gene (Sumo) of Saccharomyces cerevisiae as a purification/solubility enhancement cassette. BsaI sites are included in the cloning polylinker for scarless cloning and the production of a native, mature protein after cleavage from the Sumo fusion by Sumo protease (Ulp1).
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Utilize BL21DE3 for protein expression.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    3CLpro
  • Species
    Rhinovirus A2
  • GenBank ID
  • Promoter T7 lac
  • Tag / Fusion Protein
    • 6xHis, Sumo (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Genscript synthesized with codon optimization for E. coli

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Related to PDB: 1CQQ

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15Sumo2-HRV2-3CLpro_GTM_001 was a gift from Gaetano Montelione (Addgene plasmid # 254044 ; http://n2t.net/addgene:254044 ; RRID:Addgene_254044)
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