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pMVS1111a_Ptet_alsSD
(Plasmid #254143)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254143 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pMVS-V1
  • Backbone size w/o insert (bp) 9202
  • Total vector size (bp) 11617
  • Modifications to backbone
    including anhydrotetracycline-inducible expression system TetR/Ptet
  • Vector type
    Replicative expression plasmid for archaeon Methanothermobacter thermautotrophicus
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    acetolactate synthase
  • Alt name
    alsS
  • Alt name
    locus_tag = ST4052_04355
  • Species
    Streptococcus thermophilus
  • Insert Size (bp)
    1677
  • Mutation
    codon-optimized for Methanothermobacter thermautotrophicus
  • GenBank ID
    CP065476.1
  • Promoter PhmtB with tetO1

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    acetolactate decarboxylase
  • Alt name
    alsD
  • Alt name
    locus_tag = ST4052_04360
  • Alt name
    budA
  • Species
    Streptococcus thermophilus
  • Insert Size (bp)
    723
  • Mutation
    codon-optimized for Methanothermobacter thermautotrophicus
  • GenBank ID
    CP065476.1

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMVS1111a_Ptet_alsSD was a gift from Bastian Molitor (Addgene plasmid # 254143 ; http://n2t.net/addgene:254143 ; RRID:Addgene_254143)

  • For your References section:

    Metabolic engineering of Methanothermobacter thermautotrophicus DeltaH for recombinant acetoin production. Baur T, Allaart MT, Zipperle A, Contreras G, Fink C, Angenent LT, Molitor B. Metab Eng Commun. 2026 Mar 18;22:e00275. doi: 10.1016/j.mec.2026.e00275. eCollection 2026 Jun. 10.1016/j.mec.2026.e00275 PubMed 41940165
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