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pUG-amdSYM
(Plasmid #254144)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254144 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pUG6
  • Backbone manufacturer
    Güldener U, Heck S, Fiedler T, Beinhauer J, Hegemann JH. Nucleic Acids Research 1996; 24, 2519-2524
  • Backbone size w/o insert (bp) 4009
  • Total vector size (bp) 4807
  • Modifications to backbone
    The coding sequence of the A. nidulans amdS gene, codon-optimized for expression in S. cerevisiae and flanked by the A. gossypii TEF2 promoter and terminator, was cloned into the vector pUG6 by replacing the KanMX gene, resulting in the plasmid pUG-amdSYM
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    acetamidase
  • Alt name
    amdS cDNA
  • Species
    Aspergillus nidulans
  • Insert Size (bp)
    1646
  • GenBank ID
    M16371
  • Promoter TEF promoter from Ashbya gossypii

Cloning Information

  • Cloning method Gene Synthesis
  • 5′ sequencing primer GGGGGATCCATGCCACAATCTTGGGAAGAA
  • 3′ sequencing primer GGGCTCGAGTTATGGAGTAACAAC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUG-amdSYM was a gift from Jean-Marc Daran (Addgene plasmid # 254144 ; http://n2t.net/addgene:254144 ; RRID:Addgene_254144)

  • For your References section:

    amdSYM, a new dominant recyclable marker cassette for Saccharomyces cerevisiae. Solis-Escalante D, Kuijpers NG, Bongaerts N, Bolat I, Bosman L, Pronk JT, Daran JM, Daran-Lapujade P. FEMS Yeast Res. 2013 Feb;13(1):126-39. doi: 10.1111/1567-1364.12024. Epub 2012 Dec 17. 10.1111/1567-1364.12024 PubMed 23253382
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