pBlueSKMimRNA_CleanCap
(Plasmid #254219)

• Purpose: Template for in vitro transcription of Minos transposase using TriLink's CleanCap AG reagent. The initiator sequence of T7 promoter has been mutated (GGG to AGG).
• Depositing Lab: Amro Hamdoun
• Publication: Minos Transposase with CleanCap (unpublished)

Backbone

• Vector backbone: pBluescript SK II+
• Backbone size w/o insert (bp): 2870
• Total vector size (bp): 4773
• Modifications to backbone: T7 promoter initiator sequence has been mutated (GGG to AGG) for compatibility with TriLink's CleanCap AG reagent.
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Minos transposase
• Alt name: Tc1/mariner minos
• Insert Size (bp): 1903
• Promoter: T7

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: GTAAAACGACGGCCAGT

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Plasmid derived from pBlueSKMimRNA (Addgene Plasmid #102535) deposited by Michalis Averof.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry