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APEX2-LPETG in pET-Duet-1
(Plasmid #254276)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254276 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET-Duet-1
  • Backbone size w/o insert (bp) 5384
  • Total vector size (bp) 6191
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    APEX2
  • Alt name
    ascorbate peroxidase 2
  • Species
    Synthetic
  • Insert Size (bp)
    807
  • Tag / Fusion Protein
    • His tag (N terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Purchase from Genscript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    APEX2-LPETG in pET-Duet-1 was a gift from Mia Huang (Addgene plasmid # 254276 ; http://n2t.net/addgene:254276 ; RRID:Addgene_254276)

  • For your References section:

    Cell Surface Engineering Enables Surfaceome Profiling. Vilen Z, Reeves AE, O'Leary TR, Joeh E, Kamasawa N, Huang ML. ACS Chem Biol. 2023 Apr 21;18(4):701-710. doi: 10.1021/acschembio.1c00865. Epub 2022 Apr 20. 10.1021/acschembio.1c00865 PubMed 35443134
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