pGEM-T_hmx2
(Plasmid
#254516)
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PurposePartial hmx2 cDNA. For in vitro transcription of ISH probe
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254516 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepGEM-T
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3000
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Vector typeZebrafish Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehmx2
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Alt nameNM_001115098.4
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)630
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Entrez Genehmx2
- Promoter T7
Cloning Information
- Cloning method Other
- 5′ sequencing primer M13 fwd
- 3′ sequencing primer M13 rev
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEM-T_hmx2 was a gift from Virginie Lecaudey (Addgene plasmid # 254516 ; http://n2t.net/addgene:254516 ; RRID:Addgene_254516) -
For your References section:
Vgll4 Proteins limit Organ Size in Zebrafish through Yap1-Dependent and -Independent Mechanisms. Lardennois A, Dingare C, Duda V, Klemmt PA, Heinzen C, Kleinhans D, Falk T, Schelmbauer C, Mozolewska O, Papadopoulou S, Lai JJK, Stainier DYR, Lecaudey V. bioRxiv 2025.05.08.652796 10.1101/2025.05.08.652796