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px459-sgPNPLA3
(Plasmid #255036)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255036 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pSpCas9(BB)-2A-Puro (PX459) V2.0
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CRISPR Cas9 guide for PNPLA3 exon 3
  • gRNA/shRNA sequence
    TGGTATGTTCCTGCTTCATC
  • Species
    H. sapiens (human)
  • Promoter U6

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    px459-sgPNPLA3 was a gift from Stephen Duncan (Addgene plasmid # 255036 ; http://n2t.net/addgene:255036 ; RRID:Addgene_255036)

  • For your References section:

    A PNPLA3-Deficient iPSC-Derived Hepatocyte Screen Identifies Pathways to Potentially Reduce Steatosis in Metabolic Dysfunction-Associated Fatty Liver Disease. Doueiry C, Kappler CS, Martinez-Morant C, Duncan SA. Int J Mol Sci. 2024 Jul 2;25(13):7277. doi: 10.3390/ijms25137277. 10.3390/ijms25137277 PubMed 39000384
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