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pET28a-6xHis-SSF1
(Plasmid #255298)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255298 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET28a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SSF1
  • Species
    H. sapiens (human)
  • Entrez Gene
    PPAN (a.k.a. BXDC3, SSF, SSF-1, SSF1, SSF2)
  • Promoter T7
  • Tag / Fusion Protein
    • 6x-His (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.03.01.640913 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-6xHis-SSF1 was a gift from Richard Kriwacki (Addgene plasmid # 255298 ; http://n2t.net/addgene:255298 ; RRID:Addgene_255298)

  • For your References section:

    Granular component sub-phases direct ribosome biogenesis in the nucleolus. Dogra P, Ferrolino MC, Khatun S, Tolbert M, Miao Q, Pruett-Miller SM, Pitre A, Tripathi S, Campbell GE, Bajpai R, Freyaldenhoven T, Gibbs E, Park C-G, Kriwacki RW. bioRxiv 2025.03.01.640913 10.1101/2025.03.01.640913
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