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pYS108
(Plasmid #255530)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255530 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET28a(+)
  • Total vector size (bp) 5815
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    W163(5HTP) variant of human γS-crystallin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    590
  • Mutation
    Tryptophan 163 changed to 5-hydroxytryptophan
  • Entrez Gene
    CRYGS (a.k.a. CRYG8, CTRCT20)
  • Promoter T7

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYS108 was a gift from Rachel Martin (Addgene plasmid # 255530 ; http://n2t.net/addgene:255530 ; RRID:Addgene_255530)

  • For your References section:

    Mimicking oxidative damage in gammaS-crystallin with site-specific incorporation of 5-hydroxytryptophan. Seo Y, Long ZG, Demerdjian TK, Avenido AA, Butts CT, Martin RW. Biophys Rep (N Y). 2026 Mar 11;6(1):100251. doi: 10.1016/j.bpr.2026.100251. Epub 2026 Jan 16. 10.1016/j.bpr.2026.100251 PubMed 41548873
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