pAAV-gfaABC1D-Crimson CAAX-NES IRES Cre
(Plasmid #255734)

• Purpose: Astrocytic expression of Cre recombinase and Red Fluorescent Protein (Crimson) for labelling fine structures in astrocytes
• Depositing Lab: Thomas Oertner
• Depositing Organization: Universitaetsklinikum Hamburg-Eppendorf
  Located in Germany
• Publication: Differential roles of astrocytes and microglia in shaping ATP release events (unpublished) (unpublished)

Backbone

• Vector backbone: pAAV
• Backbone manufacturer: Stratagene
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Crimson CAAX
• Insert Size (bp): 799
• Promoter: gfaABC1D Addgene Plasmid #52925
• Tag / Fusion Protein:
  • CAAX (C terminal on insert) (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SgsI (not destroyed)
• 5′ sequencing primer: TTGCCTTTCTCTCCACAGGT
• 3′ sequencing primer: GCGGCTTCGGCCAGTAACG

Gene/Insert 2

• Gene/Insert name: IRES Cre
• Species: Synthetic
• Insert Size (bp): 1630
• Promoter: gfaABC1D Addgene Plasmid #52925

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: SgsI (AscI) (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: TTGCCTTTCTCTCCACAGGT
• 3′ sequencing primer: GTTGATTATCGATAAGCTTG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: A portion of this plasmid was derived from a plasmid made by Michael Lin (pCDNA3.1-caggs-Crimson-CAAX ,Addgene Plasmid #190041), Karl Deisseroth (pAAV-Ef1a-mCherry-IRES-Cre, Addgene Plasmid # 55632), and Baljit Khakh (pZac2.1 gfaABC1D-cyto-GCaMP6f, Addgene Plasmid #52925).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry