Skip to main content
Addgene
Open Menu

pCI-Syn-LSSmOrange
(Plasmid #255757)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255757 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    pCI
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3780
  • Total vector size (bp) 4501
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LSSmOrange
  • Species
    Synthetic
  • Insert Size (bp)
    721
  • Mutation
    A44V/F84L/W145M/I165D/M167L/G202D compared to mOrange (but numbering relative to EGFP)
  • Promoter hSyn

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer GGTCGTGAGGCACTGGGCAG
  • 3′ sequencing primer GTCCAAACTCATCAATGTATC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    A portion of this plasmid was derived from pBAD-LSSmOrange (Addgene plasmid #37129), a gift from Vladislav Verkusha.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.04.09.717511 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-Syn-LSSmOrange was a gift from Thomas Oertner (Addgene plasmid # 255757 ; http://n2t.net/addgene:255757 ; RRID:Addgene_255757)
Related items: