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Ec dnaX(gamma-tau)-pET(3c)
(Plasmid #255907)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255907 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET-3c
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    dnaX
  • Alt name
    Ec dnaX
  • Species
    E. coli

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Ec dnaX gene native sequence, which is in this plasmid, has a frameshift that happens with approximately 50% efficiency, resulting in approximately equal expression of the gamma and tau subunits of E. coli DNA Polymerase III from this clone.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ec dnaX(gamma-tau)-pET(3c) was a gift from Michael O'Donnell (Addgene plasmid # 255907 ; http://n2t.net/addgene:255907 ; RRID:Addgene_255907)
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