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pCEP4-Fab1F4-Light-chain
(Plasmid #257995)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 257995 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pCEP4
  • Backbone manufacturer
    Thermo
  • Backbone size w/o insert (bp) 10886
  • Total vector size (bp) 11654
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    anti-human-GABAA-alpha1 Fab 1F4 light chain
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    729
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFLAG tag (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GACGTCAATGGGAGTTTGTTTTG
  • 3′ sequencing primer TTTACGGTCTTTAAAAAGGCCGTA
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

We purchased the pCEP4 vector from Thermo, and synthesized the insert.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEP4-Fab1F4-Light-chain was a gift from Ryan Hibbs (Addgene plasmid # 257995 ; http://n2t.net/addgene:257995 ; RRID:Addgene_257995)
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