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pET-28a(+)-TlGlcK
(Plasmid #258836)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 258836 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET-28a(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6695
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ADP-dependent Glucokinase
  • Species
    Thermococcus litoralis
  • Insert Size (bp)
    1446
  • GenBank ID
    Q7M537.1
  • Promoter T7 lac
  • Tag / Fusion Protein
    • MGRGSHHHHHHGMA (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Azenta Life Sciences constructed the plasmid from their gene synthesis service.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-28a(+)-TlGlcK was a gift from Edward D'Antonio (Addgene plasmid # 258836 ; http://n2t.net/addgene:258836 ; RRID:Addgene_258836)

  • For your References section:

    Novel Tetrazolium-Based Colorimetric Assay for Helicase nsp13 in SARS-CoV-2. Pham, T.M.; Howard, M.G.; Carey, S.M.; Baker, L.R.; D’Antonio, E.L.. BioChem 2024, 4, 115-125. 10.3390/biochem4020006
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