pET-28a(+)-SC2Hel
(Plasmid #258844)

• Purpose: Expression of His-tagged SARS-CoV-2 Helicase nsp13 in E. coli
• Depositing Lab: Edward D'Antonio
• Publication: et al BioChem 2024, 4, 115-125.

Backbone

• Vector backbone: pET-28a(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 7097
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Helicase
• Alt name: Nonstructural Protein 13
• Alt name: nsp13
• Species: Severe acute respiratory syndrome coronavirus 2 (Wuhan-Hu-1 Strain)
• Insert Size (bp): 1848
• GenBank ID: YP_009725308.1
• Promoter: T7 lac
• Tags / Fusion Proteins:
  • MGRGSHHHHHHGMA (N terminal on insert)
  • KLAAALEHHHHHH (C terminal on backbone)

Cloning Information

• Cloning method: Gene Synthesis
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminal

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Azenta Life Sciences constructed the plasmid from their gene synthesis service.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry