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pAAV-hSyn-FRed-U6gRsGABAd-a4
(Plasmid #258849)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 258849 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 2896
  • Total vector size (bp) 6304
  • Vector type
    Mammalian Expression, AAV, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gRNA1, gRNA2, gRNA3, gRNA4, FusionRed
  • gRNA/shRNA sequence
    gRNA1 GGCTGTCCTACAACCATACCA; gRNA2 GCTTATCCGCCTACAGCCTGA; gRNA3 AGTTTAAACGAATCCCCAGGA; gRNA4 TTATGACAACAGGCTGCGTC
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Gabrd
  • Entrez Gene
    Gabra4 (a.k.a. Gabra-4)
  • Promoter U6 & Synapsin

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer TCGTGTCGTGCCTGAGAGCG
  • 3′ sequencing primer CCAGCTTGGTTCCCAATAGA
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.06.17.732978 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hSyn-FRed-U6gRsGABAd-a4 was a gift from D. James Surmeier (Addgene plasmid # 258849 ; http://n2t.net/addgene:258849 ; RRID:Addgene_258849)

  • For your References section:

    Cholinergic interneuron control of GABAergic circuits targeting spiny projection neurons is disrupted in parkinsonian models. Belal M, Perez-Rosello T, Guven EB, Kocaturk S, Xie Z, Ilijic E, Tkatch T, Li J, Dauer W, Assous M, Tepper JM, Clarke VRJ, Surmeier DJ. bioRxiv 2026.06.17.732978 10.64898/2026.06.17.732978
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