pLH31
(Plasmid
#259567)
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PurposeRMCE exchange vector containing a synthetic mini switch alpha fragment
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 259567 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepLH28
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Vector typeCre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameA synthetic fragment containing 14 repeats of the consensus IgH switch alpha 80mer
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SpeciesSynthetic
- Promoter N/A
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLH31 was a gift from Kefei Yu (Addgene plasmid # 259567 ; http://n2t.net/addgene:259567 ; RRID:Addgene_259567) -
For your References section:
Altered kinetics of nonhomologous end joining and class switch recombination in ligase IV--deficient B cells. Han L, Yu K. J Exp Med. 2008 Nov 24. 205(12):2745-53. 10.1084/jem.20081623 PubMed 19001141