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pLH31
(Plasmid #259567)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259567 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pLH28
  • Vector type
    Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    A synthetic fragment containing 14 repeats of the consensus IgH switch alpha 80mer
  • Species
    Synthetic
  • Promoter N/A

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLH31 was a gift from Kefei Yu (Addgene plasmid # 259567 ; http://n2t.net/addgene:259567 ; RRID:Addgene_259567)
  • For your References section:

    Altered kinetics of nonhomologous end joining and class switch recombination in ligase IV--deficient B cells. Han L, Yu K. J Exp Med. 2008 Nov 24. 205(12):2745-53. 10.1084/jem.20081623 PubMed 19001141