Hs HA-RTL8B
(Plasmid
#260098)
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PurposeExpression of Human 2x HA-tagged RTL8B
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Depositing Lab
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Depositing OrganizationUniversity of Colorado, Boulder
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260098 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRTL8B
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Alt namepAMW108
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SpeciesH. sapiens (human)
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Insert Size (bp)411
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Entrez GeneRTL8B (a.k.a. CXX1c, FAM127C, MAR8B, SIRH4)
- Promoter CMV
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Tag
/ Fusion Protein
- 2x HA (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Hs HA-RTL8B was a gift from Alexandra Whiteley (Addgene plasmid # 260098 ; http://n2t.net/addgene:260098 ; RRID:Addgene_260098) -
For your References section:
The gag-like gene RTL8 antagonizes PEG10-mediated virus like particles. Campodonico W, Mohan HM, Huynh PT, Black HH, Lau CI, Paulson HL, Sharkey LM, Whiteley AM. PLoS One. 2024 Dec 30;19(12):e0310946. doi: 10.1371/journal.pone.0310946. eCollection 2024. 10.1371/journal.pone.0310946 PubMed 39775359