pPCRGAL25
(Plasmid
#260268)
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PurposeAzotobacter transformation vector Avin_51320
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Depositing Lab
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Depositing OrganizationUniversity of Minnesota
Located in the United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260268 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepUC19
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Streptomycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDeoxyviolacein Operon
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SpeciesAzotobacter vinelandii
- Promoter native
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAzotobacter vinelandii. Deoxyviolacein segment taken from BBa_J72214_BBa_J72090 (Addgene plasmid #40782, a gift from Christopher Anderson).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPCRGAL25 was a gift from Brett Barney (Addgene plasmid # 260268 ; http://n2t.net/addgene:260268 ; RRID:Addgene_260268)