pMBE1
(Plasmid
#260675)
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Purpose(Empty Backbone) binary cloning vector
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Depositing Lab
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Depositing OrganizationUniversity of Alberta
Located in Canada -
Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260675 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepPK2
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Modifications to backboneHygromycin B resistance gene replaced by multiple cloning site between gpdA promoter and TtrpC terminator. Benomyl resistance gene added downstream of TtrpC terminator.
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Vector typeFungal expression
- Promoter gpdA promoter
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Selectable markersbenomyl
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMBE1 was a gift from John Vederas (Addgene plasmid # 260675 ; http://n2t.net/addgene:260675 ; RRID:Addgene_260675)
Map uploaded by the depositor.
