pSVE Grb2 myc 49L/203R
(Plasmid
#26087)
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Depositing Lab
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Depositing OrganizationWhitehead Institute for Biomedical Research
Located in the United States of America -
Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26087 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 26087-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 26087-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepSVE
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGrb2 49L/203R
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Alt nameGrb2
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SpeciesH. sapiens (human)
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Insert Size (bp)650
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Mutation49L/203R
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Entrez GeneGRB2 (a.k.a. ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, NCKAP2)
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Tag
/ Fusion Protein
- myc (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pBABE-3
- 3′ sequencing primer M13 reverse?
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
mutagenized with oligos from pSVE Grb2 myc 49L and pSVE Grb2 myc 203R together. Plasmid also known as pSVEGrb2myc u5j .
DNA (Catalog # 26087-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 26087-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSVE Grb2 myc 49L/203R was a gift from Bob Weinberg (Addgene plasmid # 26087 ; http://n2t.net/addgene:26087 ; RRID:Addgene_26087) -
For your References section:
Association of Sos Ras exchange protein with Grb2 is implicated in tyrosine kinase signal transduction and transformation. Egan SE, Giddings BW, Brooks MW, Buday L, Sizeland AM, Weinberg RA. Nature. 1993 May 6. 363(6424):45-51. 10.1038/363045a0 PubMed 8479536