pcIP22-L
(Plasmid #260929)

• Purpose: (Empty Backbone) One of the plasmids used in the chimeric-repressor bacterial two-hybrid assay. Facilitates fusion of the gene of interest to the repressor fragment cIP22.
• Depositing Lab: Jo-Anne Dillon
• Depositing Organization: University of Saskatchewan
  Located in Canada
• Publication: Chimeric-Repressor B2H Linker Plasmids (unpublished)

Backbone

• Vector backbone: pC132
• Backbone size (bp): 3557
• Vector type: Bacterial Expression
• Promoter: Plac
• Tag / Fusion Protein:
  • cIP22 N-term (N terminal on insert)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Di Lallo, Castagnoli, Ghelardini, and Paolozzi

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

One of two plasmids used in a chimeric-repressor bacterial two-hybrid assay to investigate protein-protein interactions. Use with pcI434-L (Plasmid #260930).
This plasmid was originally constructed in Di Lallo et al., 2001 (https://doi.org/10.1099/00221287-147-6-1651) and then modified by the Dillon laboratory to introduce additional restriction sites at the MCS.