SPRIZ_HA_pcDNA3.1_neo
(Plasmid #262592)

• Purpose: Mammalian expression of mouse SPRIZ (CCDC127) with a C-terminal HA tag.
• Depositing Lab: Luca Pellegrini
• Depositing Organization: University of Ottawa
  Located in Canada
• Publication: SPRIZ (CCDC127) protein conserved in deuterostome animals is a rigid OMM-IMM tether that locally sets mitochondrial IMS volume (unpublished)

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ccdc127
• Alt name: SPRIZ
• Alt name: CCDC127
• Species: M. musculus (mouse)
• Insert Size (bp): 780
• GenBank ID: NM_001168659.1
• Entrez Gene: Ccdc127 (a.k.a. AI256405, AW050060, A430066A18, C130002N06, 0610011N22Rik, 5430420E17Rik)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Mus musculus Ccdc127; RefSeq NM_001168659.1

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pcDNA3.1(+) reference: https://www.addgene.org/vector-database/2093/ ; gene reference: https://www.ncbi.nlm.nih.gov/gene/67433/