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pSMT3-CopiaGAG-His-SUMO
(Plasmid #262836)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 262836 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pSMT3-derived vector
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CopiaGAG
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    6873
  • GenBank ID
    X54147
  • Promoter T7 RNA Polymerase
  • Tag / Fusion Protein
    • His-SUMO (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer Unknown
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSMT3-CopiaGAG-His-SUMO was a gift from Brian Kelch (Addgene plasmid # 262836 ; http://n2t.net/addgene:262836 ; RRID:Addgene_262836)

  • For your References section:

    Capsid transfer of the retrotransposon Copia controls structural synaptic plasticity in Drosophila. M'Angale PG, Lemieux A, Liu Y, Wang S, Zinter M, Alegre G, Simkin A, Budnik V, Kelch BA, Thomson T. PLoS Biol. 2025 Feb 18;23(2):e3002983. doi: 10.1371/journal.pbio.3002983. eCollection 2025 Feb. 10.1371/journal.pbio.3002983 PubMed 39964983
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