Skip to main content
Addgene
Open Menu

pLenti CMV GFP MCS (721-1)
(Plasmid #26288)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26288 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
  • Backbone size (bp) 7458
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3, 37oC.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    no insert
  • Alt name
    Enhanced Green Fluorescent Protein

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eco RI (not destroyed)
  • 3′ cloning site Kpn I (not destroyed)
  • 5′ sequencing primer CMVforw
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The multiple cloning site can be used to insert either a drug selection cassette or a promoter and either a cDNA or shRNA/miRNA. The GFP can be used to visualize or isolate transduced cells.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti CMV GFP MCS (721-1) was a gift from Eric Campeau (Addgene plasmid # 26288)
Related items: