Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37a, 46]
(Plasmid
#26569)
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Depositing Lab
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Depositing OrganizationBrown University
Located in United States of America -
Publication
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Citations
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26569 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 26569-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 26569-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA6/V5-His ABC
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5100
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10F'
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Growth instructionsTop10F'
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Copy numberUnknown
Gene/Insert
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Gene/Insert namecalcium channel alpha-1B subunit
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SpeciesR. norvegicus (rat)
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Insert Size (bp)7390
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MutationDeletion of exons a10, 18a, and 24a. Contains exons 31a, 37a, and 46.
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GenBank IDAF055477
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Entrez GeneCacna1b (a.k.a. BIII)
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Tags
/ Fusion Proteins
- V5 (C terminal on backbone)
- 6XHis (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site EcorV (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH-rev
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Isolated from superior cervical ganglia.
For more information about this plasmid: http://neuroscience.brown.edu/LipscombeLab/homepage/otherpages/clonespage/clonespage1.htm
Also see: GENBANK AF222337
DNA (Catalog # 26569-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 26569-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37a, 46] was a gift from Diane Lipscombe (Addgene plasmid # 26569 ; http://n2t.net/addgene:26569 ; RRID:Addgene_26569) -
For your References section:
Cell-specific alternative splicing increases calcium channel current density in the pain pathway. Bell TJ, Thaler C, Castiglioni AJ, Helton TD, Lipscombe D. Neuron. 2004 Jan 8. 41(1):127-38. 10.1016/S0896-6273(03)00801-8 PubMed 14715140
Map uploaded by the depositor.
