pRS3 (CK2alpha, CK2beta)
(Plasmid #27092)

• Depositing Lab: David Litchfield
• Depositing Organization: University of Western Ontario
  Located in Canada
• Publication: Turowec et al Methods Enzymol. 2010;484:471-93.

Backbone

• Vector backbone: pBI
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4358
• Vector type: Mammalian Expression ; tetracycline-regulated expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: CK2beta
• Alt name: CSNK2B, G5A, CK2β, CK2N, CSK2B, MGC138222, MGC138224
• Alt name: Casein Kinase II beta
• Species: H. sapiens (human)
• Entrez Gene: CSNK2B (a.k.a. CK2B, CK2N, CSK2B, Ckb1, Ckb2, G5A, POBINDS)
• Promoter: bidirectional tet-responsive promoter
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NsiI (not destroyed)
• 5′ sequencing primer: NA
• 3′ sequencing primer: EBV-rev

Gene/Insert 2

• Gene/Insert name: CK2alpha
• Alt name: CSNK2A1, CKII, CK2A1
• Alt name: CK2α
• Alt name: Casein Kinase II alpha
• Species: H. sapiens (human)
• Entrez Gene: CSNK2A1 (a.k.a. CK2A1, CKII, Cka1, Cka2, OCNDS)
• Promoter: bidirectional tet-responsive promoter
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI (not destroyed)
• 3′ cloning site: ApaI (not destroyed)
• 5′ sequencing primer: NA
• 3′ sequencing primer: Bglob-intron-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 27092-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 27092-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.