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pCMV4-HA-NEMO
(Plasmid #27560)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27560 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMV4
  • Backbone manufacturer
    Dr. David Russell, UTSW
  • Backbone size w/o insert (bp) 4874
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NEMO
  • Species
    H. sapiens (human)
  • Mutation
    S8R
  • Entrez Gene
    IKBKG (a.k.a. AMCBX1, EDAID1, FIP-3, FIP3, Fip3p, IKK-gamma, IKKAP1, IKKG, IMD33, IP, IP1, IP2, IPD2, NEMO, SAIDX, ZC2HC9)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this is the exact plasmid (including mutation) used in the associated publication listed below.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV4-HA-NEMO was a gift from Shao-Cong Sun (Addgene plasmid # 27560 ; http://n2t.net/addgene:27560 ; RRID:Addgene_27560)
  • For your References section:

    IKKgamma serves as a docking subunit of the IkappaB kinase (IKK) and mediates interaction of IKK with the human T-cell leukemia virus Tax protein. Harhaj EW, Sun SC. J Biol Chem. 1999 Aug 13. 274(33):22911-4. 10.1074/jbc.274.33.22911 PubMed 10438454