|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27853||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2601
Vector typeEntry vector
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Growth instructionsE. coli TOP10
Copy numberLow Copy
Gene/Insert nameWRKY transcription factor from DT939730
Insert Size (bp)693
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13 For
- 3′ sequencing primer M13 Rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This CDS was amplified using PCR from a full length cDNA template from the Maize Full-length cDNA Project (www.maizecdna.org), and the PCR product included the stop codon. The PCR product was cloned into a Gateway pENTR/SD vector.
Further information on this gene may be obtained at www.grassius.org using the GenBank number. (The second half of the plasmid name is the GenBank Number for the sequence of the insert)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUT1757-HQ858771 was a gift from John Gray & Erich Grotewold (Addgene plasmid # 27853 ; http://n2t.net/addgene:27853 ; RRID:Addgene_27853)