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DsRed-p62
(Plasmid #28024)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 28024 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDsRed2-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p62
  • Alt name
    SQSTM1
  • Alt name
    sequestosome
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1070
  • Entrez Gene
    SQSTM1 (a.k.a. A170, DMRV, FTDALS3, NADGP, OSIL, PDB3, ZIP3, p60, p62, p62B)
  • Tag / Fusion Protein
    • DsRed (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer DsRed1-C
  • 3′ sequencing primer SV40-pA-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DsRed-p62 was a gift from Qing Zhong (Addgene plasmid # 28024)
  • For your References section:

    Keap1 facilitates p62-mediated ubiquitin aggregate clearance via autophagy. Fan W, Tang Z, Chen D, Moughon D, Ding X, Chen S, Zhu M, Zhong Q. Autophagy. 2010 Jul 22. 6(5):. 10.4161/auto.6.5.12189 PubMed 20495340