Flag-TRIM24-F869D
(Plasmid
#28141)
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Depositing Lab
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Depositing OrganizationUniversity of Texas MD Anderson Cancer Center
Located in the United States of America -
Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 28141 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 28141-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 28141-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCMX-(Flag)x2
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Backbone manufacturerBarton Lab
- Backbone size w/o insert (bp) 4500
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTRIM24
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SpeciesH. sapiens (human)
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Insert Size (bp)3200
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MutationA489V, F869D; aa 308 and 309 mutated for shRNA rescue
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Entrez GeneTRIM24 (a.k.a. PTC6, RNF82, TF1A, TIF1, TIF1A, TIF1ALPHA, hTIF1)
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Tag
/ Fusion Protein
- FLAGX2 (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer 5'-GTCACACCACAGAAGTAAGGT-3'
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that Addgene's sequencing results identified a A489V mutation present in TRIM24 sequence. This mutation was not intentional, but the depositing laboratory states that it does not affect plasmid function.
DNA (Catalog # 28141-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 28141-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Flag-TRIM24-F869D was a gift from Michelle Barton (Addgene plasmid # 28141 ; http://n2t.net/addgene:28141 ; RRID:Addgene_28141)