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pCAX APPs-695-alpha
(Plasmid #30147)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 30147 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCAX
  • Backbone size w/o insert (bp) 1380
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Amyloid Precursor Protein - soluble
  • Alt name
    APPs-alpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1700
  • Mutation
    amino acids 1–612
  • Entrez Gene
    APP (a.k.a. AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAX APPs-695-alpha was a gift from Dennis Selkoe & Tracy Young-Pearse (Addgene plasmid # 30147 ; http://n2t.net/addgene:30147 ; RRID:Addgene_30147)
  • For your References section:

    A critical function for beta-amyloid precursor protein in neuronal migration revealed by in utero RNA interference. Young-Pearse TL, Bai J, Chang R, Zheng JB, LoTurco JJ, Selkoe DJ. J Neurosci. 2007 Dec 26. 27(52):14459-69. 10.1523/JNEUROSCI.4701-07.2007 PubMed 18160654