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pGL3-pomMx1
(Plasmid #30536)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 30536 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 30536-D50 50 μg of DNA in Tris buffer $495
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DNA 30536-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pGL3-Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 6469
  • Total vector size (bp) 7089
  • Modifications to backbone
    Backbone is pGL3-Basic from promega. A G418 resistance cassette was amplified from the pCMV-Script (Strategene) plasmid, inserted by blunt-end ligation at the filled in BamHI site of pGL3-Basic.
  • Vector type
    Mammalian Expression, Luciferase
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Growth instructions
    There is a G418 resistance cassette giving Kanamycin resistance in prokaryotes and G418 resistance in eukaryotes.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rainbow trout Mx 1 promoter
  • Species
    Oncorhynchus mykiss
  • Insert Size (bp)
    620
  • GenBank ID
    AF310254

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer RVP3
  • 3′ sequencing primer GLP2
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The G418 resistance cassette originates from pCMV-Script (Stratagene).

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-pomMx1 was a gift from Bertrand Collet & Christopher Secombes (Addgene plasmid # 30536 ; http://n2t.net/addgene:30536 ; RRID:Addgene_30536)

  • For your References section:

    An Mx1 promoter-reporter system to study interferon pathways in rainbow trout. Collet B, Boudinot P, Benmansour A, Secombes CJ. Dev Comp Immunol. 2004 Jun . 28(7-8):793-801. 10.1016/j.dci.2003.12.005 PubMed 15043947
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