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(Plasmid #31070)


Item Catalog # Description Quantity Price (USD)
Plasmid 31070 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    Modified pEGFP-N1 (see note)
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
  • Species
    H. sapiens (human)
  • Entrez Gene
    SPTBN1 (a.k.a. DDISBA, ELF, HEL102, SPTB2, betaSpII)
  • Tag / Fusion Protein
    • HA (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This construct DOES NOT contain EGFP- the EGFP has been removed from the pEGFP-N1 backbone.

The following "mutations" have been located in this construct. The Bennett lab believes they are due to incorrect sequence data originally entered into NCBI; however, please be aware of these in case you are designing primers or doing mutagenesis:

Derivations from published sequence:

2.59 c-->t (silent)
4.23 c-->g (H-->D)
5.99 g-->c (R-->T)
6.3 g-->a (silent)

Please refer to the Addgene NGS result for the fully validated sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    beta2-spectrin-HA was a gift from Vann Bennett (Addgene plasmid # 31070 ; ; RRID:Addgene_31070)