pBabe EGFR ASVinsertion
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32066||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5169
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)3600
MutationInsert duplicate A767-V769
Entrez GeneEGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA)
- Cloning method Restriction Enzyme
- 5′ cloning site SnaB1 (destroyed during cloning)
- 3′ cloning site SnaB1 (destroyed during cloning)
- 5′ sequencing primer pBABE 5' (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBabe EGFR ASVinsertion was a gift from Matthew Meyerson (Addgene plasmid # 32066 ; http://n2t.net/addgene:32066 ; RRID:Addgene_32066)
For your References section:Allele-dependent variation in the relative cellular potency of distinct EGFR inhibitors. Yuza Y, Glatt KA, Jiang J, Greulich H, Minami Y, Woo MS, Shimamura T, Shapiro G, Lee JC, Ji H, Feng W, Chen TH, Yanagisawa H, Wong KK, Meyerson M. Cancer Biol Ther. 2007 May . 6(5):661-7. 10.4161/cbt.6.5.4003 PubMed 17495523