|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32988||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerR. Freeman/ S. Sokol
- Backbone size w/o insert (bp) 3000
Vector typeRNA Synthesis and Injections
Growth in Bacteria
SpeciesX. laevis (frog)
Insert Size (bp)1600
Entrez Genewnt2b-b (a.k.a. Xwnt-2, Xwnt-2b, Xwnt2, int1l1, irp, wnt-2, wnt-2b, wnt13, wnt2, wnt2-A, xwnt2b)
- Promoter T7
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (destroyed during cloning)
- 3′ cloning site EcoRV (destroyed during cloning)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Addgene quality control sequencing discovered a P159A point mutation. According to the depositing lab, this mutation is not a concern, as the product is functionally active.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Xwnt2b-pXT7 was a gift from Sergei Sokol (Addgene plasmid # 32988 ; http://n2t.net/addgene:32988 ; RRID:Addgene_32988)
For your References section:Xwnt-2b is a novel axis-inducing Xenopus Wnt, which is expressed in embryonic brain. Landesman Y, Sokol SY. Mech Dev. 1997 May;63(2):199-209. 10.1016/S0925-4773(97)00041-5 PubMed 9203142
Map uploaded by the depositor.